Journal: Journal of Cellular and Molecular Medicine
Article Title: Notch3 expression in capillary pericytes predicts worse graft outcome in human renal grafts with antibody‐mediated rejection
doi: 10.1111/jcmm.17325
Figure Lengend Snippet: Expression of Notch3 in normal grafts (A), and in grafts with antibody‐mediated rejection (ABMR) (B–D). (A) In normal kidneys, expression of Notch3 was mainly found to vascular smooth muscle cells (white arrows). No staining was found in peritubular capillaries. (B, C) During ABMR, a strong expression of Notch3 was observed in pericytes of peritubular capillaries (black arrows) closely attached to the endothelial cells (see inserts in the upper left and lower right corners for B and C, respectively). (D) Double staining with the vascular endothelial marker CD34 (red) and Notch3 (green) indicated that Notch3 was closely attached to and around the capillary endothelial cells in the biopsies with ABMR
Article Snippet: The human brain vascular pericytes (HBVPs) cell line (ScienCell) was cultured in pericyte medium (ScienCell) supplied with 100 U/ml penicillin/streptomycin (Gibco) on poly‐l‐lysine coated flasks according to the manufacturer's instructions.
Techniques: Expressing, Staining, Double Staining, Marker